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分享！ATCC細(xì)胞常見問題及解決方案
發(fā)布時間： 2022-10-26 點擊次數(shù)： 1112次ATCC細(xì)胞通常保存在凍存管中用干冰運輸，或是在培養(yǎng)瓶中常溫運輸。但對于國內(nèi)客戶，由于運輸時間稍長，都是采用干冰運輸?shù)姆绞健Ｔ谑盏紸TCC細(xì)胞后，很重要的一點是迅速解凍使其立即復(fù)蘇并除去DMSO，然后將它們放置到培養(yǎng)基中。如果做不到上面這點，需將細(xì)胞存儲在液氮中（-130℃以下）。不要將凍存細(xì)胞儲存在高于-130℃的溫度下，這會使細(xì)胞存活率迅速下降。ATCC細(xì)胞常見問題及解決方案：細(xì)胞漂浮：培養(yǎng)瓶不開封，瓶口酒精擦拭后平躺放置在培養(yǎng)箱。次日觀察，如細(xì)胞大部分又貼回瓶底，表明細(xì)胞活力正常，剩余漂浮的細(xì)胞可以離心去掉，留10ml培養(yǎng)液培養(yǎng)觀察，細(xì)胞生長至匯合度80%，進(jìn)行消化傳代；如細(xì)胞還是不貼壁，將細(xì)胞離心收集轉(zhuǎn)到新培養(yǎng)瓶，原培養(yǎng)瓶加部分培養(yǎng)液繼續(xù)培養(yǎng)，中間注意觀察，我們的技術(shù)人員會一直跟蹤技術(shù)指導(dǎo)，直到問題解決。客戶收到細(xì)胞后請務(wù)必仔細(xì)閱讀細(xì)胞注意事項，確保細(xì)胞的培養(yǎng)條件，如果由于培養(yǎng)條件不符導(dǎo)致細(xì)胞出現(xiàn)問題，責(zé)任由客戶自行承擔(dān)。由于運輸?shù)那闆r，所以個別細(xì)胞會出現(xiàn)不穩(wěn)定，客戶收到細(xì)胞后務(wù)必第1時間和我們，告知細(xì)胞具體情況，以便我們技術(shù)人員能及時有效的和老師溝通，不勝感謝！注意事項：1、培養(yǎng)基于4℃條件下可保存3-6個月。2、在細(xì)胞培養(yǎng)過程中，請注意保持無菌操作。3、傳代培養(yǎng)過程中，胰酶消化時間不宜過長，否則會影響細(xì)胞貼壁及其生長狀態(tài)。4、如果您的實驗時間較長，請在收到細(xì)胞后傳代時，按細(xì)胞量分種2瓶，使用其中的一瓶細(xì)胞實驗，其余的細(xì)胞先凍存起來。下次實驗需要時，再復(fù)蘇。5、該ATCC細(xì)胞，培養(yǎng)技術(shù)只可用于科研。
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